Recent Developments
Pgdıs Hands-On Workshop On Pgd For Genetıc And Chromosomal Dısorders By Trophectoderm Bıopsy
1.05.2020 00:39:29
May 12 Arrival and Accommodation
MAY 13
09:00-10:05 PGD for Single Gene and Chromosomal Disorders by Blastomere and Trophectoderm Biopsy - Theoretical Session 1
08:30 - 09:00 Registration
09:00 - 09:05 Opening of Workshop, Semra KAHRAMAN, Memorial Hospital ART and
Genetics Center, Istanbul, Turkey
09:05 - 09:35 Practical PGD, Anver KULIEV, RGI, Chicago, USA
09:35 -10:05 Advantages and disadvantages of trophectoderm biopsy - Murat CETİNKAYA, Huseyin TAC, Memorial Hospital ART and Genetics Center, Istanbul, Turkey
10:05 -10.35 DISCUSSION & COFFEE BREAK
10:35 -13:10 Hands-on Applications of Biopsy Procedures under Supervision of Trainers Participants will be divided into 3 groups, up to 5 in each for practical sessions.
- Zona Drilling Methods
- Blastomere Biopsy Techniques
- Trophectoderm Biopsy Techniques
13:10 -14:20 LUNCH & DISCUSSION
14:20 -18:30 Each group will apply all the demonstrated techniques
MAY 14
09:00 - 10:00 PGD for Single Gene and Chromosomal Disorders by Blastomere and Trophectoderm Biopsy- Theoretical Session 2
09:00 - 09:20 Clinical aspects of successful PGD, Semra KAHRAMAN, Memorial Hospital ART and Genetics Center, Istanbul, Turkey
09:20 - 09:40 PGD for Aneuploidy and Translocations- From FISH to Next Generation Technologies,TBA,Memorial Hospital ART and Genetics Center, Istanbul, Turkey
09:40 - 10:00 Molecular Techniques in PGD: Testing for Single Gene Disorders, HLA Typing, and 24 Chromosome Aneuploidy, Don LEIGH, Sydney, Australia
10:00 -10:30 DISCUSSION & COFFEE BREAK
10:30 -13:30 Hands-on applications of trophectoderm biopsy by participants
under supervision of trainers
13:30 -14:30 LUNCH & DISCUSSION
14:30 -18:30 Hands-on Applications of FISH and PCR techniques under
supervision of trainers
- Preparation, Spreading and Fixation of Blastomere and Trophectoderm Cells - In Situ Hybridization with Different Chromosome Specific Probes
- Analysis and Scoring of FISH Signals
- 1st and 2nd Round PCR for PGD of Single Gene Disorders and HLA Typing- Testing for 24 Chromosomes by Microarray Technology and NGS
Participants will be divided into 3 groups, up to 5 in each for practical sessions according to their interests.
MAY 15
09:00 -13:00 Interpreting FISH, aCGH and PCR Results
09:00 - 12:00 Analysis and Scoring of FISH Signals,and aCGH results, Don LEIGH, Syndey, Australia and Cumhur EKMEKCİ Memorial Hospital ART and Genetics Center, Istanbul, Turkey Analysis and Interpretation of NGS and PCR Results, Don LEIGH, Syndey, Australia and Cumhur EKMEKCİ Memorial Hospital ART and Genetics Center, Istanbul, Turkey
12:00 -13:00 GENERAL DISCUSSION & CLOSING
Practical Stations
Station 1: Blastomere Biopsy Techniques;
Zona hatchings by enzymatic, mechanical and laser applications
Station 2: Trophectoderm Biopsy Techniques;
Station 3: FISH Techniques;
Preparation, spreading and fixation of Blastomere and Trophectoderm Cells In situ hybridization of chromosomes with different probes
Analysis and Scoring of FISH signals
Station 4: array CGH and NGS Techniques;
Extraction and Amplification of blastomere and trophectoderm cells Labeling
Hybridization
Scanning and analysis of aCGH and NGS results Station 5: PCR Techniques;
Lysis of blastomere and trophectoderm cells
First and second round PCR for PGD of single gene disorders and HLA matching Mutation Analysis and Restriction Enzyme Digestion Analysis and interpretation of PCR results
- These applications will be done simultaneously in 4 stations and each participant will attend to each station and perform all demonstrated techniques